10 Objectives for Week 3
- Express entire set of wild-type and ncAA-mutant proteins.
- Purify wild-type protein from cells harvested in Week 2.
- Make storage and assay buffers.
- Desalt the wild-type protein sample into the storage buffer.
- Analyze purified protein by SDS-PAGE.
- Determine if protein is active.
- Continue literature search for an appropriate and feasible assay to test enzyme kinetics.
- Obtain chemicals and supplies for assays.