Consider which location and which ncAAs will be incorporated/studied based on literature searches and the Pymol image. Identify sites that have already been mutated. Finalize selection of which protein you will study this term and begin to develop hypothesis for term-long projects.
Set up group bench and calibrate and sterilize pipettes.
Obtain instructions on expressing protein from the plasmid-containing cells.
Ensure that the necessary media component solutions and sterile equipment are prepared and available for expressions during week 2 (wild-type protein) and week 3 (set of wild-type and ncAA-mutant proteins).