10 Objectives for Week 3

  • Express entire set of wild-type and ncAA-mutant proteins.
  • Purify wild-type protein from cells harvested in Week 2.
  • Make storage and assay buffers.
  • Desalt the wild-type protein sample into the storage buffer.
  • Analyze purified protein by SDS-PAGE.
  • Determine if protein is active.
  • Continue literature search for an appropriate and feasible assay to test enzyme kinetics.
  • Obtain chemicals and supplies for assays.

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Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual Copyright © 2019 by Ryan Mehl, Kari van Zee & Kelsey Keen is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, except where otherwise noted.