Appendix 6: List of Available Sites, Non-canonical Amino Acids, and Plasmids
| Available Expression Plasmids | Protein Encoded | Antibiotic Resistance | Addgene ID |
| pBad CA native | CA thermostable wild-type | ampicillin | 105665 |
| pBad CA TAG20 | CA thermostable TAG20 | ampicillin | 105666 |
| pBad CA TAG93 | CA thermostable TAG93 | ampicillin | 105667 |
| pBad CA TAG97 | CA thermostable TAG97 | ampicillin | 105668 |
| pBad CA TAG126 | CA thermostable TAG126 | ampicillin | 105836 |
| pBad CA TAG186 | CA thermostable TAG186 | ampicillin | 105837 |
| pBad CA TAG233 | CA thermostable TAG233 | ampicillin | 105838 |
| pBad HPII native | Catalase HPII wild-type | ampicillin | 105839 |
| pBad HPII TAG206 | Catalase HPII TAG206 | ampicillin | 105846 |
| pBad HPII TAG392 | Catalase HPII TAG392 | ampicillin | 105848 |
| pBad HPII TAG415 | Catalase HPII TAG415 | ampicillin | 105847 |
| pBad HPII TAG568 | Catalase HPII TAG568 | ampicillin | 105845 |
| pBad HPII TAG283 | Catalase HPII TAG283 | ampicillin | 105843 |
| pBad HPII TAG348 | Catalase HPII TAG348 | ampicillin | 105844 |
| pBad sfGFP | sfGFP | ampicillin | 85482 |
| pBad sfGFP TAG150 | sfGFP TAG150 | ampicillin | 85483 |
CA: human carbonic anhydrase II thermostable
HPII: E. coli HPII catalase
sfGFP: superfolder green fluorescent protein
| Enzyme | TAG Site | ncAAs for Incorporation |
| CA
Human thermostable |
F20 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| F93 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| W97 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| K126 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| E186 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| E233 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| HPII
E. coli |
F206 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| E283 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| K348 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| H392 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| Y415 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| D568 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| GCE Plasmid | Machinery | Compatible ncAAs for Incorporation | Antibiotic Resistance | Addgene ID |
| pDule-para-aminoPhe | pAF | pAF | tetracycline | 85502 |
| pDule-3-nitrotyrosine | NitroY | 3-NY | tetracycline | 85498 |
| pDule-pCNF | pCNF | pAzF, p-NO2F, pBrF, pIF, O-CH3Y | tetracycline | 85494 |
ncAA Abbreviations:
- 3-NY: 3-nitrotyrosine
- pAzF: para-azidophenylalanine
- p-NO2F: para-nitrophenylalanine
- pBrF: para-bromophenylalanine
- pAF: para-aminophenylalanine
- pIF: para-iodophenylalanine
- O-CH3Y: O-methyltyrosine (synonym: 4-methoxy-phenylalanine)
