Primary Navigation
Book Contents Navigation
Cover
1. Background
2. Overview of Laboratory Responsibilities Tentative Schedule
3. Objectives for Week 1
4. Concepts for Choosing and Expressing Mutants
5. Objectives for Week 2
6. Concepts for Protein Expression
7. Methods for Protein Expression
8. Concepts for SDS-PAGE
9. Methods for SDS-PAGE
10. Objectives for Week 3
11. Concepts for Protein Purification and Desalting
12. Methods for Protein Purification and Desalting
13. Microfluidizer Lysis Method
14. Objectives for Week 4
15. Methods for Protein Standard Curves and SDS-PAGE
16. Objectives for Weeks 5-9
17. Concepts for Enzyme Assays
18. Methods for Enzyme Assays
Appendix
Appendix 1: Micropipette Calibration
Appendix 2: Genetic Sequences
Appendix 3: Structures of Available Non-canonical Amino Acids
Appendix 4: Non-Inducing and Autoinduction Media
Appendix 5: Master Supply List
Appendix 6: List of Available Sites, Non-canonical Amino Acids, and Plasmids
Creative Commons License
Recommended Citations
Versioning
(Note: Not all structures are available for every site of every enzyme)
Previous/next navigation
Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual Copyright © 2019 by Ryan Mehl, Kari van Zee & Kelsey Keen is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, except where otherwise noted.